PH control

[wilsonjholmes]

Has anyone implemented any form of PH control with the chi.bio system? My university got a couple units and are wanting to look into retrofitting units to do PH control. I am thinking an i2c board with a BNC connector so that a probe can be connected and incorporated into the software. Looking for suggestions on where and what to start looking for.

[harrison]

Hi Wilson
This is something I have been interested in, but have not yet got around to building. I think the major question is the probe – what probe and how does it fit into the test tube. Do you have one that you want to use? I am happy to help on the software/hardware front.
Harrison

[wilsonjholmes]

Testing posting with a different browser.

[wilsonjholmes]

Testing with another browser…

[wilsonjholmes]

One of the professors I am working with first suggested this one (https://www.hannainst.com/fc240b-small-diameter-foodcare-ph-electrode-with-stainless-steel-body.html).

[wilsonjholmes]

…but then got back with me later with a different one (https://www.hannainst.com/hi1093b-ph-electrode-with-micro-bulb-and-extended-length.html) as the first one was meant for semisolids and would not be applicable to the research she is conducting.

[wilsonjholmes]

The probe she suggested uses a BNC connector so it should be able to be used with the Atlas EZO board (https://atlas-scientific.com/circuits/ezo-ph-circuit/) and a BNC connector. It is also only 3mm in diameter so it should be able to fit in the vial easily I think.

[harrison]

I think this looks like an interesting project and I’d be glad to help. I agree that the Atlas Scientific solution seems elegant, low cost, and I would be happy to make an adapter PCB to integrate this with the existing Chi.Bio.

The major challenge I foresee is mounting the PH probe itself in the device’s test tube – it seems the default probe from Atlas is quite large. They do have some smaller versions: https://atlas-scientific.com/probes/
Such as their micro pH probe: https://atlas-scientific.com/probes/micro-ph-probe/
But they are also quite expensive.

What kind of probe were you thinking of using? There are others offered by companies such as ThermoFisher:
https://www.fishersci.com/shop/products/orion-9810bn-micro-ph-electrode/13299511
Though they are even more expensive.

Harrison

[wilsonjholmes]

Was looking into this probe (a professor said that this one would work for their/our application):
https://www.hannainst.com/hi1093b-ph-electrode-with-micro-bulb-and-extended-length.html

[wilsonjholmes]

I am not sure if I fully understand the different specifications of these probes outside of their pH range, physical size, and build materials.
Are there more things that I should be looking at?
I have been referencing this website to learn more about the probes as I have not worked with them before:
https://www.ysi.com/ysi-blog/water-blogged-blog/2019/02/anatomy-of-ph-electrodes

[harrison]

Certainly, different probes may be more accurate than others. However, I would focus right now on getting one that is able to easily integrate (i.e. physically fit!) within the test tubes you are using. Probably your application doesn’t need absolutely the best pH precision, right? More important is that it is timely, effective etc…

[harrison]

I will have a look at well – currently we are investigating implementing the Atlas PCB you linked previously.

[wilsonjholmes]

Was looking into this probe (a professor said that this one would work for their/our application):
https://www.hannainst.com/hi1093b-ph-electrode-with-micro-bulb-and-extended-length.html
I am not sure if I fully understand the different specifications of these probes outside of their pH range, physical size, and build materials.
Are there more things that I should be looking at?
I have been referencing this website to learn more about the probes as I have not worked with them before:
https://www.ysi.com/ysi-blog/water-blogged-blog/2019/02/anatomy-of-ph-electrodes

[wilsonjholmes]

One of the professors I am working with first suggested this one (https://www.hannainst.com/fc240b-small-diameter-foodcare-ph-electrode-with-stainless-steel-body.html) to me, but then got back with me later with another one (https://www.hannainst.com/hi1093b-ph-electrode-with-micro-bulb-and-extended-length.html)

[harrison]

Both of those look promising, we could fairly easily integrate either into the Chi.Bio. Was your plan to have this combine with the Atlas sensor PCB?

[Author]

Posts