Lids leaking?

[Julia]

Hello,
we are encountering some kind of leakage of the chemostats. I am pretty sure they are not overflowing (we took all precautions, making sure all pumps seal well and the medium is below the reactors. After the reactors stop they are not wet on the top but inside the reactor and the vials still contain the accurate volume). Has anybody encountered that the lids are leaking on the sides? Any fix (we tried parafilm to no avail)? It stops us from running the reactors for more than 6-8 hours. Any suggestions are welcome.

Thanks a lot.

[harrison]

Hello Julia,

We did actually also have this issue recently, which we think is down to a change in 3d printing technology used to manufacture the lids.
We solved it by putting O-rings on the underside of the lids. This is the part we are using:
https://uk.rs-online.com/web/p/gaskets-o-rings/1965666
But anything similar should work. Important quantities are the internal diameter (external can be a little different and still fine), and material (such that it is autoclavable).

We found that putting these in our lids and screwing them down tight fixed the issues.

Harrison

[Julia]

Thanks a lot for the quick reply. Immediately ordered some o-rings. Hopefully this will fix the issue.

Best,
Julia

[harrison]

Good stuff. You should be able to fit them around the circular raised part of approximately 15mm diameter underneath the lid. Might require use of some tweezers to get it well seated on the lid’s upper surface.
Harrison

[Author]

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