Hi. Sorry if this question has been answered elsewhere but I could not find a clear response.
The fluorescence that chi.bio measures is somehow normalized by OD? In other words, the output is a fluorescence/cell value or the overall fluorescent signal of the culture? Specifically, we are measuring the fluorescence of an mCherry constitutively expressed in E. coli (starting OD = 0.1) and we are letting the culture grow from OD=0.1 to OD=1. Should we expect the signal to increase together with OD (as in the case of a measured cumulative fluorescence) or should we expect the signal to remain constant (as in the case of measured fluorescence/cell)? Also, the emit FP2 values are in the range of 1.6 while in most of the documentation of chi.bio typical value are in the order of 0.01 or even lower. Is this indicative of something strange?
Thanks in advance for your assistance.
best
m.