Hi Dr. Steel,
Thank you for the prompt reply! The inoculum was from a culture in its stationary phase– are you saying that for bacteria constitutively producing GFP the amount of GFP per cell might be higher at this phase? And to be clear, the culture started at a very high dilution (i.e. no detectable OD/fluorescence) so presumably we’re seeing the bacteria re-entering the exponential phase pretty quickly, are we not?
Re: the high OD behavior, are you suggesting theres a max OD (seen at around the 17 hour mark) after which the baseband signal does not increase with OD due to the degree of scatter?
Yes we will be working with a fixed OD, was just trying to understand what we were seeing prior to this! Especially considering the normalized FP signal is at some points lower than the starting (pretty much blank media) signal.. want to make sure the readout we’re seeing is in fact true fluorescence!
