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  • in reply to: How were the laser calibration factors for OD determined #1788
    Noelia
    Participant

    Hi there,

    To get the raw measurements in the ChiBio do I need to set LEDFa=1? (I’m doing it with scattered light). I’m doing this to calculate LEDFa with my cells and media through a curve calibration and I don’t want the default calibration to interefere.
    Same for absorbance, should I set Lasera / Laserb differently?

    Thanks,

    Noelia

    in reply to: OD meausured via scattering as opposed to absorbance #1786
    Noelia
    Participant

    Hi both,

    I was able to do what is recommended here. I have the calibration curve for different ODs measured both in the ChiBio and spectrophotometer. Is this calibration curve external? Or can I add it somehow in the code so the OD is automatically calculated in the ChiBio?

    Thanks!

    Noelia

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