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  • Kate
    Participant

    Ok awesome, thanks. I’m still unclear about how, once the calibration has been done to provide an initial OD (which seems to be the lines 1572-76), which parameter in the code captures subsequent raw measurements, and how these are scaled using the calibration to create the final OD readings that are saved in the output file?

    in reply to: Reactor Volume Control #1685
    Kate
    Participant

    Ok yes thanks that helps. So stirring can affect the volume pumped out for a given pump rate? i.e. potentially marginally more volume removed than if stirring was turned off? Our stir rate is set to 0.6 so shouldn’t be stirring for a full cycle, but I guess it depends if the stirring and pumps overlap (e.g. they both initiate at the beginning of each cycle and are therefore always running at the same time?).

    For one of our applications it’s fairly important to have a good handle on volume regulation as we’re adding a fixed amount of product to the system without pumps on (but always stirred), leaving it for a while and the using the pumps to wash through fresh media. We therefore need to calculate the dilution rate / how many cycles it will take to wash the product out using the pumps at a given level. Thanks!

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