[jeff8838]

Okay scratch that – I figured out how things were computed 🙂

[jeff8838]

Also, how did you derive the following equation in the code?
Do you use the following equations: OD0 = ODRaw/ODActual, and ODi = log10(OD0/ODRaw), and the general quadratic equation for ax^2 + bx + c = 0?

raw=((ODActual/a + (b/(2*a))**2)**0.5) – (b/(2*a)) #THis is performing the inverse function of the quadratic OD calibration.
OD0=(10.0**raw)*ODRaw

[jeff8838]

Hey there,

A follow-up question: is the “a” variable (sysData[M][‘OD0’][‘LASERa’]) similar to the 0.3974 term in front of ODi in equation (S2) of the supplementary paper, and is the “b” variable (sysData[M][‘OD0’][‘LASERb’]) the same as the 1.374 term in front of ODi^2 in equation (S2)?

[jeff8838]

Hi Harrison,

Would an example of “blank media” for the computation of Mo be water?

Thanks

[jeff8838]

Thank you – I found the information I was looking for in the Chi.Bio paper.

[jeff8838]

Great, thanks for the information.

[jeff8838]

Thanks, yeah – I was referring to the current through the LED.

Do you use a static power setting when performing OD measurements? Is there an optimal power setting that we should use for OD measurements?

Thanks!

[jeff8838]

Hi Harrison,

Some follow-up questions:

1. We’ve noticed that most OD600 meters seem to only support an OD up to 2.5A or 3.0A. What would you suggest doing if we want to calibrate above 2.5 or 3.0?

2. In the supplemental information document, you mention that one calibration solution you could use is evaporated milk. What OD value should evaporated milk have?

Thanks for your help!

[jeff8838]

Hi Harrison,

Another question: what OD range does the Chi.Bio platform support?

[jeff8838]

Thanks, that clarifies things.

[jeff8838]

Great, thanks for the explanations Harrison!

I’ll let you know if I have any further questions.

[Author]

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