Hi John and Harrison,
I can comment on our experience with yeast using light-induced expression mNeonGreen (single integrated copy of the promoter, full induction around pTDH3 strong promoter). We can clearly see the fluorescence reading change when switching ON or OFF the induction for a single culture in turbidostat mode. But the signal is low, so raw numbers cannot be compared between reactors. Also the OD matters a lot, even a small ‘dither’ will be visible on the reading. To be able to compare data between different runs and different reactors, we are now testing if a single calibration (specific to a given reactor, OD and media obviously) provides week-to-week reproducibility.
François
