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  • in reply to: Problem with OD calibration #1097
    david020506
    Participant

    Dear Harrison,

    I had time to look again at this problem and it now works. I replaced the old app.py and cb.sh in my ChiBio (which came built-in in the device) for the current version of the files in https://github.com/HarrisonSteel/ChiBio. It seemed that the previous version of the app.py had some bug.

    Thanks for your help!

    Cheers

    in reply to: Problem with OD calibration #968
    david020506
    Participant

    Dear Harrison,

    Thank you so much for taking the time to answer my question.

    I do the following:

    1. Click measure.
    2. Read a value of 13271 from next to the word “Raw”
    3. Copy this value (13271) into the box “Measured” which in right of the “Actual” Box. There is no “NEW” box, except for the one above the “Measure” box, which sets a Target OD for regulation.
    4. Put the actual OD (lets say 0.01) into “Actual”. Here if I put 0,the current value always changes to 0.
    5. Click “Set”
    6. Current changes to 100000, not 13271 as expected. In fact, any value larger than 30 sets Current to 100000.

    I started playing around with the value I introduce in the “Measured” box and found that for an “Actual” OD of 0.01,the “Measured” value should be something like 23 in order to make “Current” change to 13271. When doing this, measured values displayed in the OD chart during the experiment indeed start at 0.01 and increase to reasonable values during cultivation, although they don’t match the OD measured in our spectrometer.

    Any suggestions are welcomed =) Thanks a lot again!

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