Thanks for the reply!
In order to use Chi.Bio in fed-batch mode, what do you think would be the easiest approach?
The idea would be to run a batch phase until glucose is completely consumed (all pumps switched off in batch phase, no feeding at all). We already have some data on the time it takes the strains to consume all the glucose, so probably we could use experiment time as trigger signal to turn on pump1 and start feeding. The goal is to follow an exponential feeding profile (F = F0 * e^(k*t), where F0 is the initial feeding rate, k is a constant and t is the feeding time), but I’m not sure how easy it would be to establish that in a custom program. We would of course need to estimate feeding rates for each of the pump1 as I’ve read quite some variation can be expected, but I’m not sure if we could face additional issues. Maybe if we reach high cell densities oxygen becomes a limitation too?
Regarding sampling we could just stick a syringe through the lid, even though it could be time-consuming for many samples I believe it’s still doable.
