[anon1234567]

Hi Harrison, thanks for that, it is working now ! I am measuring with the OD LEDA (395nm). With regards to a calibration curve my thought process is to grow the cyano cells to a very high OD (eg 15) and then measure the OD with the Chi bios and the bench top spectrometer at 730nm. I will then dilute multiple times while measuring in the same process, to form a curve. Does that seem like a good way to calibrate the online OD measurements with the bench spectrometer?

[anon1234567]

Hi, just a follow up comment. Looking at the code I think you have already put an else if function for LEDA and LEDF in the measureOD function in App.py, with the corresponding OD calibration constants. Am I understanding this correctly and if so, would just modifying the first two ‘LASER650’ lines to ‘LEDA’or ‘LEDF’ be sufficient to measure OD via scattering?

[anon1234567]

Hi Harrison, thanks for the response. So if I understand correctly, I would change the ‘LASER650’ to one of the LED’s (eg. LED623nm). Is that all that is required as in the supplementary paper you also mention different correlations used for absorbance as opposed to scattering. Would I need to further modify the code or is what I stated above sufficient for measuring OD via scattering?

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