The stirring system can be quite sensitive if it is a large stirrer with strong magnet, FOr this reason I would strongly suggest the recommended stirrer and running it at higher speed if you need better stirring.
Pump – It could be a hardware issue, have you tried a different pump board on the same reactor? If that works, but the othe rpump doesnt, likely that pump is broken.
For the OD function, the Pump=0.02 is the maximum rate. So, this is what you might expect it to deliver if you are way above the target OD. If you are delivering this amount of pumping but you are still unable to reduce the OD of growing cells, it could be the pump is not making a good seal on the tubing or for other reasons it isn’t ACTUALLY pumping. Look at the troubleshooting guide in the manual as there are various ways to improve this, e.g. putting some tape in the pump head, or putting a one way check valve between the pumps and the reactor so liquid cant flow backward at any time.
Harrison
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