Problem with air bubbles forming inside the tubing

[harrison]

Hello,
Are we talking about the culture tubes or the silicone tubing?
If the later, one thing to do is put a one way check valve between the pump and reactor (i.e. in the fresh media line). This prevents it moving backwards under gravity/siphon.

[mgalardini]

Hi,

correct, this has to do with air bubbles forming in the silicone tubing. Do you have a particular product/supplier you would recommend?

[harrison]

Currently we use part 7757K41 from here:
https://www.mcmaster.com/products/one-way-flow-valves/check-valves~/body-material~plastic-1/check-valves-with-barbed-fittings-9/

[mgalardini]

Thanks a lot, we will try that, although we saw that the problem was somewhat reduced when we placed the media bottles well above the pumps/vials.

One last question: do these valves affect the flow, meaning that the amount of liquid being pumped per unit of time is lower?

[Author]

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