How were the calibration factor values determined in the CalibrateOD() function?

[jeff8838]

Hi there,

In the CalibrateOD() function within app.py, I see there are a number of hard-coded values depending on the M value and device (see below). How were these values determined? Just wondering how we would go about computing these calibration factor values ourselves.

For LEDF:
if (M==’M0′):
CF=1299.0
elif (M==’M1′):
CF=1206.0
elif (M==’M2′):
CF=1660.0
elif (M==’M3′):
CF=1494.0

For LEDA:
if (M==’M0′):
CF=422
elif (M==’M1′):
CF=379
elif (M==’M2′):
CF=574
elif (M==’M3′):
CF=522

Thanks!

[harrison]

I hope my answer to your other post answers this: https://chi.bio/forums/topic/questions-regarding-od-measurement/#post-1251
If I recall correctly the confusion might be arising from inconsistent parameter names on my behalf, it looks like “CF” is the same as “P0” discussed in the paper (i.e. equation S3).

[jeff8838]

Thanks, that clarifies things.

[Author]

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