Home Forums Software How were the calibration factor values determined in the CalibrateOD() function?

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  • #1250
    jeff8838
    Participant

    Hi there,

    In the CalibrateOD() function within app.py, I see there are a number of hard-coded values depending on the M value and device (see below). How were these values determined? Just wondering how we would go about computing these calibration factor values ourselves.

    For LEDF:
    if (M==’M0′):
    CF=1299.0
    elif (M==’M1′):
    CF=1206.0
    elif (M==’M2′):
    CF=1660.0
    elif (M==’M3′):
    CF=1494.0

    For LEDA:
    if (M==’M0′):
    CF=422
    elif (M==’M1′):
    CF=379
    elif (M==’M2′):
    CF=574
    elif (M==’M3′):
    CF=522

    Thanks!

    #1252
    harrison
    Keymaster

    I hope my answer to your other post answers this: https://chi.bio/forums/topic/questions-regarding-od-measurement/#post-1251
    If I recall correctly the confusion might be arising from inconsistent parameter names on my behalf, it looks like “CF” is the same as “P0” discussed in the paper (i.e. equation S3).

    #1255
    jeff8838
    Participant

    Thanks, that clarifies things.

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